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Rehydrate the sample in a reversed methanol B1N buffer series for the times indicated in table 2. 7 Picower Institute for Learning and Memory, Massachusetts Institute of Technology, Cambridge, MA, USA. Here, we describe the methods for sample preparation, pretreatment, staining, clearing, and mounting for imaging. Kuwajima T et al. SpringerBriefs in Physics. Curiosity is human nature. Chicago: The University of Chicago Press. Frontiers in Genetics. "Dyes from a twenty-first century perspective".